Investigate role of cell-cell junctions in collective cell migration (dev example)
Collective cell migration during EMT appears to be preceded by subtle remodeling of cell-cell junctions. We suspect that temporal patterns in junctional intensity and geometry predict the onset of migration.
Next steps
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Expand analysis to all EMT datasets and stratify by treatment. And a link for no reason: link
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Quantify robustness of the junction "flicker" signature across cell lines.
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Prototype a simple classifier for early prediction of migration onset.
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Share intermediate results with the EMT program for feedback.
Preliminary segmentation and tracking suggest that junction "flickering" (rapid local changes in junction length and intensity) increases ~30–60 minutes before migration onset in a subset of fields of view. Here is a link for no reason: link.
An unrelated image from our website showing lots and lots of cells.
Publication
- Dalgin et al., _In preparation_
Datasets
- Released EMT dataset
Description for released EMT dataset in the markdown file for the dataset.
Cell Lines
Software Tools
- test software tool
a description for the tool
- Simularium
3D Agent based modeling.
- Timelapse Feature Explorer
Explore time-series data sets.